The Medical University of South Carolina
Student Broadcast Messages
Find

shRNA Shared Technology Resource

(Academic)

United States
http://hcc.musc.edu/research/resources/shRNA.htm
2013-07-10
Dr David P Turner 8437928011 turnerda@musc.edu

shRNA Shared Technology Resource http://hcc.musc.edu/research/resources/shRNA.htm

Use the shRNA resource in your research efforts to quickly knockdown your gene of interest:

-The resource provides easy and affordable access to the largest genome wide human and mouse library of short hairpin RNA (shRNA) vectors
-shRNA inhibits target gene expression via RNA interference to prevent protein expression
-Knockdown your target gene(s) of interest easily and reliably using the most validated short hairpin libraries available
-Target single genes of gene family sets as well as pathway specific pooled libraries
-Vectors are available to all MUSC investigators at a cost of $20.00 each
-The resource will also provide protocol and troubleshooting guidance to all investigators

Further information regarding the resource can be found at: http://hcc.musc.edu/research/resources/shRNA.htm

Please contact the resource director with all questions and enquiries at:
turnerda@musc.edu

shRNA Library Features
-Largest and most validated shRNA collection
-Human Library: 20,018 genes, 129,695 clones (1500-96 well plates)
-Mouse Library: 21,171 genes, 118,062 clones (1374-96 well plates)
-Hairpins comprised of a 21mer base stem and a 6 base loop designed against NCBI REFSEQ
-Sequence, specificity & position scoring with the Broad Institute algorithm
-A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript
-For any given RefSeq, there is often a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs.

Lentiviral Vector Features:
-shRNA cloned into the pLKO vector developed by the Broad Institute
-Allows for both stable or transient transfection
-Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids
-Stable gene silencing is selected using the puromycin selectable marker
-Integrates for long-term knockdown
-Transduces virtually any cell type (dividing or non-dividing)
-No interferon response
-Lack of recombination issues

171 Ashley Avenue · Charleston SC 29425 · (843) 792-2300