MUSC shRNA Shared Resource
Dr. David P. Turner 792-8011 firstname.lastname@example.org
The MUSC shRNA Technology Resource will be open to business on Monday, April 1.
The Resource will provide all investigators at MUSC access to genome wide human and mouse libraries that together encode a total of almost 160,000 shRNA clones against over 41,000 genes. The resource utilizes The RNAi Consortium's (TRC) genome-wide lentiviral mouse and human libraries and investigators will have the option of ordering shRNA's targeting single or multiple genes, gene family sets as well as pathway specific pooled libraries. This technology holds tremendous power, and is ready to help investigators at MUSC work toward breakthrough discoveries.
To provide investigators with detailed information regarding the libraries themselves and their potential use, you are invited to attend a seminar by Philip Gibbs, Field Applications Specialist for Sigma-Aldrich Corporation titled:
Modulation of Gene Expression Using Lentivirus-Based RNA Interference and Other Small RNAs
Abstract: Technologies that allow researchers to routinely modulate the expression level of virtually any gene within a species have greatly enhanced the understanding of gene function. The most common method of altering gene expression is at the RNA level in the form of RNA interference (RNAi). Sigma is a leading provider of RNAi tools for gene silencing, such as shRNA, miRNA, siRNA, and esiRNA. The MISSION? shRNA portfolio from Sigma consists of the world's largest collection of shRNA clones with the highest coverage of genes across the human and mouse genome. These highly validated lentiviral-based shRNA libraries from The RNAi Consortium (built by the Broad Institute at Harvard and MIT) have been widely used for the study of individual genes as well as for screening applications. During the seminar we will discuss the use of shRNA, arrayed and pooled format, to study protein function and to identify novel proteins in any disease or pathway.