The Proteomics of Shrimp & Oyster Innate Immunity
The Schey lab is interested in understanding innate immunity in marine invertebrates. More specifically, we are examining how shrimp (Pacific white shrimp, Litopenaeus vannamei) respond to pathogenic or environmental challenge and isolating antimicrobial peptides in oysters. We are applying proteomics techniques to examine protein expression in challenged shrimp starting with hemolymph and gill tissues.
Litopenaeus vannamei
The Schey lab is in the process of isolating antimicrobial molecules from oyster (Eastern Oyster, Crassostrea virginica) hemolymph. Antimicrobial assays combined with mass spectrometry are used to identify and structurally characterize active compounds.
Related References | Additional Publications
Ouvry Patat SA and Schey KL. Characterization of antimicrobial histone sequences and posttranslational modifications by mass spectrometry. J. Mass Spectrom. 42:664-674, 2007.
Xue G, Itoh N, Schey KL, Li Y, Cooper RK, La Peyre JF. A New Lysozyme from the Eastern Oyster (Crassostrea virginica) Indicates Adaptive Evolution of i-Type Lysozymes in Bivalve Mollusks. Cell. Mol. Life Sci. 64:82-95, 2007.
Xue G, Waldrop GL, Schey KL, Itoh N, Ogawa M, Cooper RK, Losso JN, LaPeyre JF. A novel slow-tight binding serine protease inhibitor from eastern oyster (Crassostrea virginica) plasma inhibits protease of the oyster parasite Perkinsus marinus. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 145(1):16-26, 2006. (Epub 2006 Jun 3.)
Xue QG, Schey KL, Volety AK, Chu FLE, LaPeyre JF. Purification and characterization of lysozyme from plasma of the eastern oyster, crassostrea virginica. Comparative Biochemistry and Physiology Part B 139:11-25, 2004.
Jenny MJ, Ringwood AH, Schey KL, Warr GW, Chapman RW. Diversity of metallothioneins in the American oyster, Crassostrea virginica, revealed by transcriptomic and proteomic approaches. Eur. J. Biochem. 271:1702-1712, 2004.
Patat SA, Carnegie RB, Kingsbury C, Gross PS, Chapman R, Schey KL. Antimicrobial activity of histones from hemocytes of the Pacific white shrimp. Eur. J. Biochem. 271(23-24):4825-33, 2004.
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