Following are some publications by members of the mass spectrometry group:

Z. Ablonczy, R.K. Crouch, P.W. Goletz, T.M. Redmond, D.R. Knapp, J.X. Ma and B. Rohrer. 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors. J. Biol. Chem. 277(43):40491-8, 2002.

Z. Ablonczy, M. Kono, R. K. Crouch and D. R. Knapp. Mass spectrometric analysis of integral membrane proteins at the subnanomolar level: Application to recombinant photopigments. Analytical Chemistry 73:4774-4779, 2001.

J.-S. Kim and D.R. Knapp. Minaturized electrospray ionization emitters on a poly(dimethylsiloxane) microfuidic devices. Electrophoresis 22:3993-3999, 2001.

A. Gelasco, R.K. Crouch and D. Knapp. Intrahelical arrangement in the integral membrane protein rhodopsin investigated by site-specific chemical cleavage and mass spectrometry. Biochemistry 39:4907-4914, 2000.

K.L. Schey, M.L. Little, J.G. Fowler and R.K. Crouch. Characterization of human lens major intrinsic protein structure. Invest. Ophthalmol. Vis. Sci. 41:175-182, 2000.

K.L. Schey, S. Patat, C.F. Chignell, M. Datillo, R.H. Wang and J.E. Roberts. Photooxidation of lens proteins by hypericin (active ingredient in St. John's Wort). Photochem. Photobiol. 72:200-207, 2000.

W.E. McIntire, J. Dingus, K.L. Schey and J.D. Hildebrandt. Characterization of the major bovine brain G_o alpha isoforms: Mapping the structural differences between the alpha subunit isoforms identifies a variable region of the protein involved in receptor and effector interactions. J. Biol. Chem. 273:33135-33141, 1998.

M. Busman, K.L. Schey and D. R. Knapp. Identification of the phosphorylation sites in phosphopeptides by positive and negative mode electrospray ionization- tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 7:243-249, 1996.

M.G. Bartlett, K.L. Busch, C.A. Wells and K.L. Schey. Use of 2-hydroxy-1-naphthoic acid as a matrix for matrix-assisted laser desorption ionization mass spectrometry of low molecular weight porphyrins and peptides. J. Mass Spectrom. 31:275-279, 1996.

N.O. Artymev, M. Busman, K.L. Schey and H.E. Hamm. Mechanism of photoreceptor cGMP PDE inhibition by its g-subunits. Proc. Natl. Acad. Sci. USA. 93:5407-5412, 1996.

K.L. Schey. Hydrophobic proteins and peptides analyzed by MALDI in methods in molecular biology, Vol. 61, J.R. Chapman (ed), Humana Press, 1996.

M.D. Wilcox, J. Dingus, E.A. Balcueva, W.E. McIntire, N.D. Mehta, K.L. Schey, J.D. Robishaw and J.D. Hildebrandt. Bovine brain Go isoforms have distinct g subunit compositions. J. Biol. Chem. 270:4189-4192, 1995.

K.L. Schey, D. A. Durkin and K.R. Thornburg. Design and performance of an in-line surface induced dissociation device in a four sector mass spectrometer. J. Amer. Soc. Mass Spectrom. 6:257, 1995.

M.D. Wilcox, K.L. Schey, M. Busman and J.D. Hildebrandt. Determination of the complete covalent structure of the g 2 subunit of bovine brain G-proteins by mass spectrometry. Biochem. Biophys. Res. Commun. 212: 367-374, 1995.

R.E. Scarberry, Z. Zhang and D.R. Knapp. Peptide sequence determination from high-energy CID spectra using artificial neural networks. Journal of the American Society for Mass Spectrometry 6:947-961,1995.

M. Busman, D. R. Knapp and K.L. Schey. Observation of large multimers in the electrospray ionization-mass spectrometry of peptides. Rapid Communications in Mass Spectrometry 8:211-216, 1994.

C. J. Beischel, K. Gray, E.S. Hazard III, D. R. Knapp and R. K. Crouch. Evaluation of photaffinity labeled sites of bacteriorhodopsin using molecular modeling. Biochemistry and Molecular Biology International 32:933-939, 1994.

C. J. Beischel, D. R. Knapp, R. Govindjee, T. G. Ebrey and R. K. Crouch. Azidotetrafluorophenyl retinal analogue: synthesis and bacteriorhodopsin pigment formation. Photochemistry and Photobiology 60:64-68, 1994.

M.D. Wilcox, K.L. Schey, J. Dingus, N. Mehta, B.S. Tatum, M. Halushka, J.W. Finch and J.D. Hildebrandt. Analysis of G-protein g subunit heterogeneity using mass spectrometry. J. Biol. Chem. 269:12508, 1994.

O. Artemeyev, J. S. Mills, K. R. Thornburg, D. R. Knapp, K. L. Schey and H. E. Hamm. A site on transducin a-subunit of interaction with the polycationic region of cGMP- phosphodiesterase inhibitory subunit. J. Biol. Chem. 268:23611-23615, 1993.

T. Walle, U.K. Walle, K.R. Thornburg and K.L. Schey. Stereoselective sulfation of albuterol in humans: Biosynthesis of the sulfate conjugate by HEPG2 cells. Drug Metab. Disp. 21:76, 1993.

K. R. Thornburg, K.L. Schey and D.R. Knapp. Identification of a new fragment ion type in the collision induced dissociation spectra of peptides: the formation of a2-16 ions. J. Amer. Soc. Mass Spectrom. 4:424, 1993.

D.C. McMillan, K.L. Schey, G.P. Meier and D.J. Jollow. Structural identification and hemolytic activity of divicine. Chem. Res. Toxicol. 6:439,1993.

D. R. Knapp, J. E. Oatis, Jr., and D. I. Papac. A small scale multiple peptide synthesis system; application to phosphopeptide synthesis. International Journal of Peptide and Protein Research 42:259-263, 1993.

D. I Papac, J. E. Oatis, Jr., R. K. Crouch and D. R. Knapp. Mass spectrometric identification of phosphorylation sites in bleached bovine rhodopsin. Biochemistry 32:5930-34, 1993.

J. W. Finch, R. K. Crouch, D. R. Knapp and K. L. Schey. Mass spectrometric identification of modifications to human serum albumin treated with hydrogen peroxide. Archives of Biochemistry and Biophysics 305:595-599, 1993.

D.I. Papac, K.R. Thornburg, E.E. Bullesbach, R.K. Crouch and D. R. Knapp. Palmitylation of a G-protein coupled receptor. Direct analysis by mass spectrometry. J. Biol. Chem. 267:16889-16894, 1992.

K.L. Schey, D.I. Papac, D.R. Knapp and R.K. Crouch. Matrix-assisted laser desorption of rhodopsin and bacteriorhodopsin. Biophys. J. 63:1240, 1992.

D. I. Papac, K. L. Schey and D. R. Knapp. Combination electrospray-liquid secondary ion mass spectrometry ion source. Anal. Chem. 63:1658-1660, 1991.

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We are located in room 305 of the Children's Research Institute Building at MUSC.

Biomolecular Mass Spectrometry Facility Department of Pharmacology Medical University of South Carolina 173 Ashley Avenue, CRI 305 Charleston, SC 29425	Telephone Numbers: 843-792-5849 (CRI 305) 843-792-2471 (department office) FAX: 843-792-2475