Microfluidic System for Multidimensional Proteome Analysis

Following complete sequencing of the genome, the next challenge will be to describe the repertoire of proteins synthesized by specific cell types under specific circumstances, i.e. proteome analysis. Such study will require development of analytical methodology capable of addressing very complex mixtures of proteins. This work is directed toward development of such methodology using an integrated microfluidics ("microchip") system employing three "orthogonal" separation/analysis modes (isoelectric focusing [IEF], partition chromatography, and mass spectrometry [MS]) followed by computer analysis of the resulting data files.

The proposed system will be capable of identification of proteins in complex mixtures, including posttranslationally modified proteins, and definition of quantitative differences between protein mixtures. The prototype of the proposed system will be implemented using recently developed "soft lithography" methods.

 

We are located in room 305 of the Children's Research Institute Building at MUSC.

Biomolecular Mass Spectrometry Facility Department of Pharmacology Medical University of South Carolina 173 Ashley Avenue, CRI 305 Charleston, SC 29425	Telephone Numbers: 843-792-5849 (CRI 305) 843-792-2471 (department office) FAX: 843-792-2475