MALDI Tissue Imaging & Profiling
MALDI tissue images from a bovine lens section indicating expression of alphaA- and alphaB-crystallin and their modified forms.
MALDI tissue imaging and profiling project involves state-of-the-art proteomic imaging of protein expression patterns directly from tissue samples. Imaging is distinguished from profiling by the end result being an image of specific protein abundance across a tissue (as in the figure above). Profiling simply provides protein mass spectra at specific locations on the tissue. Initial work has focused on lens protein imaging and profiling; however, the acquisition of an NIH S10 Shared Instrumentation grant will bring new capabilities to the MUSC campus applicable to a wide variety of projects.
Related References | Additional Publications
Grey AC and Schey KL. Distribution of Bovine and Rabbit Lens α-crystallin products by MALDI Imaging Mass Spectrometry. Molecular Vision, in press.
Han J and Schey KL. MALDI tissue imaging of ocular lens α-crystallin. Invest. Ophthalmol. Vis. Sci. 47:2990-6, 2006.
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