Tandem Mass Spectrometry of Intact Proteins
As part of the MUSC Cardiovascular Proteomics Center, funded by the National Heart, Lung and Blood Institute, a project is underway to develop new proteomics technology for high-throughput identification of proteins by tandem mass spectrometry. A state-of-the-art tandem time-of-flight instrument (Applied Biosystems 4700 Proteomics Analyzer) is being used to fragment intact proteins. An algorithm has been developed to very rapidly search fragmentation patterns against protein databases and identify proteins. In the future, custom tandem time-of-flight instruments will be constructed to enhance the technology.
The Applied Biosystems 4700 Proteomics Analyzer
Relevant References | Additional Publications
Liu Z and Schey KL. Fragmentation of Multiply-Charged Intact Protein Ions Using MALDI TOF-TOF Mass Spectrometry. J. Amer. Soc. Mass Spectrom. 19:231-238, 2008. (Epub ahead of print June 2007.)
Liu Z and Schey KL. Optimization of a MALDI TOF-TOF Mass Spectrometer for Intact Protein Analysis. J. Am. Soc. Mass Spectrom. 16:482-490, 2005.
Schey KL, Busman M, Cook LA, Hamm HE, and Hildebrandt JD. Structural Characterization of Intact G-protein γ-Subunits by Mass Spectrometry. Meth. Enzymol. 344:586-597, 2002 (G Protein Pathways, Part C, ed by R Iyengar and JD Hildebrandt).
Cook LA, Wilcox MD, Dingus J, Schey KL, and Hildebrandt JD. Separation and Analysis of G Protein γ Subunits. Meth. Enzymol. 344:209-233, 2002 (G Protein Pathways, Part C, ed by R Iyengar and JD Hildebrandt).
Schey KL, Cook LA and Hildebrandt JD. Ion trap mass spectrometry of GTP-binding protein γ-subunits. Int. J. Mass Spectrom. 212:377-388, 2001.
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