Daynna Wolff, Ph.D.

Professor
Director of Cytogenetics
Department of Pathology & Laboratory Medicine
Office: Childrens Hospital Room 222C
Phone: (843) 792-3574
Fax: (843) 792-1248

CLINICAL EXPERTISE:

Clinical Cytogenetics
Molecular Genetics
Cytogenomic Microarray Analysis
Genetics of Bladder Cancer
Laboratory Quality Assurance

EDUCATION:

B.A., 1985, College of Notre Dame of Maryland, Baltimore
Ph.D., 1991, Division of Human Genetics, University of Maryland at Baltimore
Post-Doctoral Fellowship, 1992, Department of Genetics, Stanford University
1993-1995, Clinical Cytogenetics and Clinical Molecular Genetics Fellow, Center for Human Genetics, Cleveland, Ohio

RESEARCH INTERESTS:

Genetics of Bladder Cancer
Cytogenomic microarray analysis of cancers
Oncology biomarkers

RECENT PUBLICATIONS:

1. Brothman AR, Schneider NR,, Saikevych I, Cooley LD, Butler MG, Patil S, Mascarello J, Rao K, Dewald G, Park JP, Persons D, Wolff DJ, Vance GH. Cytogenetic Heteromorphisms: Survey results and reporting practices of G-band regions that we have pondered for years. Arch Path Lab Med, 130:947-949, 2006.
2. Heaney DL, Flume P, Hamilton L, Lyon E, Wolff DJ. Detection of an apparent homozygous 3120G A cystic fibrosis mutation on a routine carrier screen. J Mol Diag 8:137-40, 2006.
3. Persons DL, Tubbs RR, Cooley LD, Dewald GW, Dowling PK, Du E, Mascarello JT, Rao KW, Wilson KS, Wolff DJ, Habegger-Vance G. HER-2 fluorescence in situ hybridization: results from the survey program of the College of American Pathologists. Arch Pathol Lab Med. 130:325-31, 2006.
4. Graf M.D., Christ L, Mascarello JT, Mowrey P, Pettenati M, Stetten G, Storto P, Surti U, Van Dyke DL, Vance GH, Wolff D, Schwartz S. Redefining the risks for prenatally ascertained supernumary marker chromosomes: A collaborative study. J Med Genet.43:660-4, 2006.
5. Quigley DI, Wolff DJ. Pediatric T-cell Acute Lymphoblastic Leukemia with Aberrations of both MLL Loci, Cancer Genetics and Cytogenetics, 168:77-79, 2006.
6. Benz CC, Fedele V, Xu F, Yistra B, Ginzinger B, Yu M, Moore D, Hall RK, Wolff DJ, Disis ML, Eppenberger-Castori S, Eppenberger U, Schittulli F, Tommassi S, Paradiso A, Scott GK, Albertson DG. Altered promoter usage characterizes monoallelic transcription arising with ERBB2 amplification in human breast cancers. Genes, Chromosomes & Cancer 45:983-994, 2006.
7. Wolff DJ, Bagg A, Cooley AD, Dewald GW, Hirsch BA, Jacky PB, Rao KW, Rao PN. Guidance for fluorescence in situ hybridization (FISH) testing in hematologic disorders. J Mol Diagn. 9:134-43, 2007 and joint publication in J Mol Diagn. 9:134-43, 2007.
8. Bilic M, Quigley DI, Stuart RK, Wolff DJ. Jumping translocation of 1q in BCR/ABL-positive acute lymphoblastic leukemia. Cancer Genet Cytogenet. 172:90-91, 2007.
9. Quigley DI, Brooker J, Wolff DJ. The Prothrombin 20209C T Sequence Variant: To Test or Not to Test? J Assoc Genet Technol. 33:1-3, 2007.
10. Znoyko I, Stuart RK, Ellingham T, Winters J, Wolff DJ, Quigley DI. Tetraploidy and 5q deletion in M.D.S: A case report. Cancer Genet Cytogenet. 183(1):64-8, 2008.
11. Wolff DJ, Heaney DL, Neuwald PD, Stellrecht KA, Press RD. Multi-Site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization: a report of the association for molecular pathology. J Mol Diagn. 11:87-92, 2009.
12. Wolff, DJ, Van Dyke, DL, Powell CM and the Working Group of the ACMG Laboratory Quality Assurance Committee. Laboratory Guideline for Turner syndrome. Genet Med. 12:52-55, 2010.
13. Zhang L Chan SSL, Wolff DJ. Mitochondrial Disorders of DNA Polymerase g Dysfunction: From Anatomic to Molecular Pathology Diagnosis. Arch Path Lab Med 135:925-934, 2011.
14. Post G, Black JS, Cortes GY, Pollack RB, Wolff DJ, Lazarchick J. The utility of fluorescence in situ hybridization (FISH) analysis in diagnosing graft versus host disease following orthotopic liver transplant. Ann Clin Lab Sci 41:188-92, 2011.
15. Brothman AR,Dolan MM, Goodman BK, Park JP, Persons DL, Saxe DF, Tepperberg JH, Tsuchiya KD, Van Dyke DL, Wilson KS, Wolff DJ, Theil KS. CAP/ACMG proficiency testing for constitutional cytogenomic microarray analysis. Genetics in Med 13:765-769, 2011.
16. Kearney HM, South ST, Wolff DJ, Lamb A, Hamosh,A, Rao KW. ACMG recommendations for the design and performance expectations for clinical genomic copy number microarrays intended for use in the postnatal setting for detection of constitutional abnormalities. Genet Med 13:676-679, 2011.
17. Zhang L, Znoyko I, Costa LJ, Conlin LK, Daber R, Self S, Wolff DJ. Clonal diversity using SNP microarrays: A new prognostic marker for chronic lymphocytic leukemia. Cancer Genet, in press.
18. Saxe DF, Persons DL, Wolff DJ, Theil KS. Validation of fluorescence in situ hybridization using an analyte-specific reagent for detection of abnormalities involving the mixed lineage leukemia gene. Ach Pathol Lab Med 136:47-52, 2012.

INVITED REVIEW MANUSCRIPTS:

1. Wolff DJ. Advances in laboratory evaluation of Turner syndrome and its variants: Beyond cytogenetics studies. Indian J Peds 67:825-830, 2000.
2. Laudadio J, Quigley DI, Tubbs R, Wolff DJ. HER2 testing: A review of detection methodologies and their clinical performance. Expert Rev Mol Diagn.7:53-64, 2007.
3. Wolff DJ. The Genetics of bladder cancer: A cytogeneticist’s perspective. Cytogenet Genome Res.118(2-4):177-81, 2007.

BOOK CHAPTERS:

1. Wolff DJ, Brothman A, Hirsch B, Jacky P. Clinical Cytogenetics. In, American College of Medical Genetics Standards and Guidelines for Clinical Genetics Laboratories, edited by M Watson, www.acmg.net, 2004.
2. Wolff DJ, Schwartz S. Clinical Applications of Fluorescence In Situ Hybridization. In, The Principles of Clinical Cytogenetics, Second Edition, edited by S.Gerson and M Keagle. Humana Press, Inc., Totowa, NJ, 2005.
3. Wolff DJ. Advances in Clinical Cytogenetics. In, Encyclopedia of Genomics, Proteomics and Bioinformatics, edited by M Dunn, L Jorde, P Little and S Subramaniam. John Wiley & Sons, Ltd., West Sussex, UK, 2006.