To serve patients, health care providers, research scientists, scholars, and society by providing excellence and innovation in diagnostic services and educational resources in a respectful, professional and culturally diverse atmosphere.
To become a preeminent leader in academic anatomic and clinical pathology while translating basic science discovery to improved clinical care.
Amanda C. LaRue, Ph.D.
Department of Pathology & Laboratory Medicine
Research Health Scientist, Research Services, VAMC
Scientific Director, HCC Flow Cytometry and Cell Sorting Shared Resource
Office: Ralph H. Johnson VA Medical Center Research Services Room 151
Phone: (843) 789-6713
Fax: (843) 876-5381
1993-1997 B.S., Biology; B.A., French; Wofford College, Spartanburg, South Carolina
1999-2003 Ph.D., Molecular, Cellular and Pathobiology, Medical University of South Carolina, Charleston, South Carolina
2003-2005 Post-doctoral Fellowship, Experimental Hematology, Ralph H. Johnson VAMC and Medical University of South Carolina Wofford College
My primary research interest lies in the plasticity of hematopoietic stem cells (HSCs). Using a unique single cell HSC transplantation model, work in my laboratory addresses the role of HSCs in both pathological and physiological conditions. Current studies are directed at two major areas. The first is the examination of the role of HSCs in the development and progression of solid tumors. Our in vivo studies have demonstrated that both circulating fibroblast precursors (CFPs) and carcinoma associated fibroblasts (CAFs) are of HSC origin. We are currently investigating the roles that these HSC-derived stromal cells play in promoting tumor progression and metastasis and elucidating mechanisms regulating the cross-talk between these HSC-derived cells and tumor cells. The long-term goal of these studies is to target HSC-derived CFPs and CAFs to inhibit tumor progression. The second area of focus for my laboratory is aimed at determining the ability of HSCs to generate mesenchymal cells including adipocytes, osteocytes and chondrocytes. Our recent data from transplantation models indicate that HSCs give rise to adipocytes, osteoblasts, osteocytes and chondrocytes in vivo. Current studies are aimed at determining the participation of these HSC-derived cells in injury models such as fracture and disorders such as obesity. The ultimate goal of these studies is to develop unique avenues of therapy for diseases of mesenchymal tissues.
Drake CJ, LaRue A, Ferrara N and Little CD (2000). VEGF regulates cell behavior during vasculogenesis. Developmental Biology, 224, 18-188. PMID: 10926758
Argraves WS, LaRue AC, Fleming PA and Drake CJ (2002). VEGF is required for the assembly but not the maintenance of embryonic blood vessels. Developmental Dynamics, 225, 298-304. PMID: 12412012
LaRue AC, Mironov VA, Argraves WS, Czirók A, Fleming PA and Drake CJ (2003). Patterning of embryonic blood vessels. Developmental Dynamics, 228, 21-29. PMID: 12950076
LaRue AC, Lansford R and Drake CJ (2003). Circulating blood island-derived cells contribute to vasculogenesis in the embryo proper. Developmental Biology, 262, 162-172. PMID: 14512026.
Drake CJ, Fleming PA, LaRue AC, Barth JL, Chintalapudi MR and Argraves WS (2004). Differential distribution of Cubilin and Megalin expression in the mouse embryo. The Anatomical Record, Part A, 277A:163-170. PMID: 14983511
LaRue AC, Zile MH, and Drake CJ (2004). Normal vascular morphogenesis requires retinol-mediated regulation of endothelial cell numbers. Developmental Dynamics, 230(4), 666-674. PMID: 15254901
Wang Y, Schulte BA, LaRue AC, Ogawa M and Zhou D. (2006). Total body irradiation selectively induces murine hematopoietic stem cell senescence. Blood, 107, 358-366. PMID: 16150936.
LaRue AC, Masuya M, Ebihara Y, Fleming PA, Visconti RP, Ogawa M, and Drake CJ (2006). Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumors. Experimental Hematology, 34, 208-218. PMID: 16459189.
Ebihara Y, Masuya M, LaRue AC, Yang S, Owens RW, Argraves K, Visconti RP, Fleming PA, Minamiguchi H, Hill WD, Martin-Studdard A, Drake CJ, and Ogawa M (2006). Hematopoietic origin of fibroblasts: II. In vitro studies of fibroblasts, CFU-F and fibrocytes. Experimental Hematology, 34, 219-229. PMID: 16459190.
Visconti RP, LaRue AC, Ebihara Y, McQuinn TC, Fleming PA, Minimaguchi H, Markwald RR, Ogawa M, and Drake CJ (2006). Hematopoietic stem cell-derived valve interstitial cells contribute to adult valve homeostasis. Circulation Research, 98, 690-6. PMID: 16456103.
Ogawa M, LaRue AC, and Drake CJ (2006). Hematopoietic Origin of Fibroblasts/Myofibroblasts: Its Patho-physiological Implications. Blood, 108, 2893-2896. PMID: 16840726.
Szalai G, LaRue AC and Watson DK (2006). Molecular Mechanisms of Megakaryopoiesis. Cellular and Molecular Life Sciences, 63, 2460-2476. PMID: 169092036.
Ogawa M and LaRue AC (2007). Origin of CFU-F. Experimental Hematology, 35(9), 1319-1320. PMID: 17637495
Zhang XK, Moussa O, LaRue AC, Bradshaw S, Molano I, Spyropoulos DD, Gilkeson GG, and Watson DK (2008). The Transcription Factor Fli-1 Modulates Marginal Zone and Follicular B cell Development in Mice. J Immunology, 181(3):1644-54. PMCID: PMC2504761.
Shirai K, Sera Y, Bulkeley W, Mehrotra M, Moussa O, LaRue AC, Watson DK, Stuart RK, Lazarchick J, and Ogawa M (2009). Hematopoietic Stem Cell Origin of Human Fibroblasts: Cell Culture Studies of Female Recipients of Gender Mismatch Stem Cell Transplantation and Patients with Chronic Myelogenous Leukemia. Experimental Hematology, 37: 1464-1471. 2009. PMCID: PMC2802494.
Sera Y, LaRue AC, Moussa O, Mehrotra M, Duncan JD, Williams CR, Nishimoto E, Schulte BA, Watson PM, Watson DK, and Ogawa M (2009). Hematopoietic Stem Cell Origin of Adipocytes. Experimental Hematology, 37: 1108-1120. 2009. PMCID: PMC2740899
Abangan RS, Williams CR, Mehrotra M, Duncan JD, and LaRue AC (2010). MCP1 Directs Trafficking of Hematopoietic Stem Cell-Derived Fibroblast Precursors in Solid Tumor. American Journal of Pathology, 176(4):1914-26.PMID: 20167869.
Ogawa M, LaRue AC, Watson PM, and Watson DK (2010). Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approaches. International Journal of Hematology, 91(3):353-9. PMID: 20336396
Mehrotra M, Rosol M, Ogawa M, and LaRue AC (2010). Amelioration of a mouse model of osteogenesis imperfecta with hematopoietic stem cell transplantation: microcomputed tomography studies. Experimental Hematology, 38(7):593-602. PMID: 20417683
Ogawa M, LaRue AC, Watson PM, and Watson DK (2010). Hematopoietic stem cell origin of connective tissues. Experimental Hematology, 38(7):540-7. PMID: 20412832
Jyothi V, Li M, Kilpatrick LA, Smythe N, LaRue AC, Zhou D, Schulte BA, Schmeidt RA, and Lang H (2010). Unmyelinated Auditory Type I Spiral Ganglion Neurons in Congenic Ly5.1 Mice. Journal of Comparative Neurology, 518(16):3254-71. PMID: 20575058
*Moussa O, *LaRue AC, Abangan RS, Williams CR, Zhang X, Masuya M, Gong YZ, Spyropoulos DD, Ogawa M, Gilkeson GG, and Watson DK (2010). Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Molecular and Cellular Biology, Sep 7, Epub ahead of print. PMID: 20823267. *OM and ACL contributed equally to this study.
Turner DP, Findlay VJ, Moussa O, Semenchenko VI, Watson PM, LaRue AC, Desouki MM, Fraig M, Watson DK (2011). Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression. Prostate. 2011. Epub 2011, Mar 28. PMID: 21446014
Findlay VJ, Turner DP, Yordy JS, McCarragher B, Shriver MR, Szalai G, Watson PM, LaRue AC, Moussa O, Watson DK (2011). Prostate-Derived ETS Factor Regulates Epithelial-to-Mesenchymal Transition through Both SLUG-Dependent and Independent Mechanisms, Genes and Cancer, 2(2):120-9. PMID: 21779485.
Mehrotra M, Williams CR, Ogawa M, and LaRue AC. Hematopoietic stem cells give rise to osteo-chondrogenic cells. Blood Cells, Molecules and Diseases, 50(1):41-9.2012. PMID: 22954476 http://dx.doi.org/10.1016/j.bcmd.2012.08.003.
McDonald LT and LaRue AC. Hematopoietic stem cell derived carcinoma-associated fibroblasts: A novel origin. International J of Clin and Exp Path, 5(9):863-73. 2012. PMID: 23119103.
Ogawa M, LaRue AC, Mehrotra M. Hematopoietic Stem Cells Are Pluripotent and Not Just Hematopoietic. Blood Cells, Molecules and Diseases, in press. 2012.
Chapters in Scholarly Books:
LaRue AC and Ogawa M (2007). Hematopoietic Origin of Fibrocytes. In Fibrocytes: New Insights into Tissue Repair and Systemic Fibroses. Editor, Richard Bucala. Yale University, USA.
Mehrotra M and LaRue AC. A therapeutic role for hematopoietic stem cells in osteogenesis imperfecta. Genetic Disorders, in press. ISBN 980-953-307-7.