Clinical Expertise:

• Molecular Cytogenetics

• Genetics of Bladder Cancer

• Laboratory Quality Assurance

Education:

• B.A., 1985, College of Notre Dame of Maryland, Baltimore

• Ph.D., 1991, Division of Human Genetics, University of Maryland at Baltimore

• Post-doctoral Fellowship, 1992, Department of Genetics, Stanford University

• 1993-1995, Clinical Cytogenetics and Clinical Molecular Genetics Fellow, Center for Human Genetics, Cleveland, Ohio

Research Interests:

1. X Inactivation

2. Molecular Cytogenetics

3. Genetics of Autism

Recent Publications:

• Christiansen LR, Lage JM, Wolff DJ, Pai GS, Harley RA. Mosaic duplication 1(q11q44) in an infant with nephroblastomatosis and mineralization of extraplacental membranes. Pediatr Dev Pathol. 8:115-23, 2005
• Christiansen L, Allen RA, Dunn T, Wolff DJ. A case of infantile AML with MLL-MLL10 fusion caused by insertion of 11q into 10p. Canc Genet Cytogenet, 159:181-183, 2005.
• Quigley DI, Wolff DJ. Cytogenetics caseload and FTE survey summary 2005. J Assoc Genet Tech, 31:59-62, 2005.
• Laudadio J, Keane TE, Reeves HM, Savage SJ, Hoda RS, Lage JM, Wolff DJ. Fluorescence in situ hybridization for detecting transitional cell carcinoma: implications for clinical practice. Brit J Urol, Internat 96:1280-1285, 2005.
• Chen B, O’Connell CD, Boone DJ, Amos JA, Beck JC, Chan MM, Farkas DH, Lebo RV, Richards CS, Roa BB, Silverman LM, Barton DE, Bejjani BA, Belloni DR, Bernacki SH, Caggana M, Charache P, Dequeker E, Ferreira-Gonzalez A, Friedman KJ, Greene CL, Grody WW, Highsmith WE, Jr., Hinkel CS, Kalman LV, Lubin IM, Lyon E, Payne DA, Pratt VM, Rohlfs E, Rundell CA, Schneider E, Willey AM, Williams LO, Willey JC, Winn-Deen ES, Wolff DJ.  Developing a sustainable process to provide quality control materials for genetic testing. Genet Med, 7:534-549, 2005.
• Skorupa A, Metzner-Sadurski J, Wolff DJ, Gupta R, Hall RK. HER-2/neu amplified breast cancers in South Carolina. J South Carolina Med Assoc., 101:97-99, 2005.
• Spector EB, Grody WW, Matteson CJ, Palomaki GE, Bellissimo DB, Wolff DJ, Bradley LA, Prior TW, Feldman G, Popovich BW, Watson MS, Richards CS. Technical standards and guidelines: Venous thromboembolism (Factor V Leiden and prothrombin 20210G>A testing): A disease-specific supplement to the standards and guidelines for clinical genetics laboratories. Genet Med. 7:444-453, 2005.
• Yu J, Kindy MS, Ellis BC, Baatz JE, Peden-Adams M, Ellingham TJ, Wolff DJ, Fair PA, Gattoni-Celli S.  Establishment of epidermal cell Lines derived from the skin of the Atlantic bottlenose dolphin (Tursiops Truncatus). The Anatomical Record, Part A, Discoveries in Molecular, Cellular & Evolutionary Biology. 287:1246-55, 2005.
• Parker R, Reeves H, Sudarshan S, Wolff D, Keane T. Abnormal in situ hybridization analysis in collecting duct carcinoma. Urology 66:1110, 2005.
• Hirsh B, Brothman AR, Jacky PB, Rao KW, Wolff DJ. Section E6 of the ACMG technical standards and guidelines: chromosome studies for acquired abnormalities. Genet Med. 7:509-513, 2005.
• Brothman AR, Schneider NR,, Saikevych I, Cooley LD, Butler MG, Patil S, Mascarello J, Rao K, Dewald G, Park JP, Persons D, Wolff DJ, Vance GH. Cytogenetic Heteromorphisms: Survey results and reporting practices of G-band regions that we have pondered for years. Arch Path Lab Med, 130:947-949, 2006.
• Heaney DL, Flume P, Hamilton L, Lyon E, Wolff DJ. Detection of an apparent homozygous 3120G>A cystic fibrosis mutation on a routine carrier screen. J Mol Diag 8:137-40, 2006.
• Persons DL, Tubbs RR, Cooley LD, Dewald GW, Dowling PK, Du E, Mascarello JT, Rao KW, Wilson KS, Wolff DJ, Habegger-Vance G.  HER-2 fluorescence in situ hybridization: results from the survey program of the College of American Pathologists. Arch Pathol Lab Med. 130:325-31, 2006.
• Graf MD, Christ L,  Mascarello JT,  Mowrey P, Pettenati M, Stetten G, Storto P, Surti U, Van Dyke DL, Vance GH, Wolff D, Schwartz S. Redefining the risks for prenatally ascertained supernumary marker chromosomes: A collaborative study. J Med Genet.43:660-4, 2006.
• Quigley DI, Wolff DJ. Pediatric T-cell Acute Lymphoblastic Leukemia with Aberrations of both MLL Loci, Cancer Genetics and Cytogenetics, 168:77-79, 2006.
• Benz CC, Fedele V, Xu F, Yistra B, Ginzinger B, Yu M, Moore D, Hall RK, Wolff DJ, Disis ML, Eppenberger-Castori S, Eppenberger U, Schittulli F, Tommassi S, Paradiso A, Scott GK, Albertson DG. Altered promoter usage characterizes monoallelic transcription arising with ERBB2 amplification in human breast cancers. Genes, Chromosomes & Cancer 45:983-994, 2006.
• Wolff DJ, Bagg A, Cooley AD, Dewald GW, Hirsch BA, Jacky PB, Rao KW, Rao PN. Guidance for fluorescence in situ hybridization (FISH) testing in hematologic disorders. J Mol Diagn. 9:134-43, 2007 and joint publication in J Mol Diagn. 9:134-43, 2007.
• Bilic M, Quigley DI, Stuart RK, Wolff DJ.  Jumping translocation of 1q in BCR/ABL-positive acute lymphoblastic leukemia. Cancer Genet Cytogenet. 172:90-91, 2007.
• Quigley DI, Brooker J, Wolff DJ. The Prothrombin 20209C>T Sequence Variant: To Test or Not to Test?  J Assoc Genet Technol. 33:1-3, 2007.
• Znoyko I, Stuart RK, Ellingham T, Winters J, Wolff DJ, Quigley DI. Tetraploidy and 5q deletion in MDS: A case report. Cancer Genet Cytogenet, in press.

Book Chapters:

• Wolff DJ, Brothman A, Hirsch B, Jacky P. Clinical Cytogenetics. In, American College of Medical Genetics Standards and Guidelines for Clinical Genetics Laboratories, edited by M Watson, www.acmg.net, 2004.
• Wolff DJ, Schwartz S. Clinical Applications of Fluorescence In Situ Hybridization. In, The Principles of Clinical Cytogenetics, Second Edition, edited by S.Gerson and M Keagle. Humana Press, Inc., Totowa, NJ, 2005.
• Wolff DJ. Advances in Clinical Cytogenetics. In, Encyclopedia of Genomics, Proteomics and Bioinformatics, edited by M Dunn, L Jorde, P Little and S Subramaniam. John Wiley & Sons, Ltd., West Sussex, UK, 2006.