Severine Patat (Pharmacology student)

Mentor: Dr. Kevin Schey

Year Graduated 2005

Degrees: MUSC 2000-present PhD. in Pharmacology
University Pierre et Marie Curie, Paris, France 1995-2000
Organic Spectroscopy DEA (Mention Bien)
Chemistry Maitrise (Mention Assez Bien)
Chemistry Licence (Mention Assez Bien)
Mathematics, Chemistry and Physics DEUG (Mention Bien)

patatsa@musc.edu
Research
Shrimp innate immunity. The goal of the project is to identify antimicrobial peptides/proteins in shrimp hemolymph. Preliminary data showed that hemocytes (shrimp blood cells) contain high levels of histone proteins. In various vertebrates, histones have been shown to have antimicrobial activity. Since hemocytes are immune effector cells in the shrimp, we hypothesized that they contained high levels of histones to fight microorganisms. Shrimp histones H2A, H2B/H4 and H1 fragment were purified and inhibited growth of the Gram positive bacteria M. luteus. Defining mechanism of action and localization of histones as well as characterizing changes in hemolymph proteome upon challenge are in progress.
 
Severine talks with Matt and Peko

HONORS AND AWARDS:
NSA (National Shellfisheries Association) travel award 2004
Dean’s Academic Scholarship 2004
Outstanding Instructor Award Nominee 2003

PROFESSIONAL SOCIETIES:
American Society for Mass Spectrometry
National Shellfisheries Association
PUBLICATIONS:

Patat SA, Carnegie RB, Kingsbury C, Gross PS, Chapman R, and Schey KL. 2004. Antimicrobial activity of histones from hemocytes of the Pacific White Shrimp. Eur. J. Biochem. 271: 4825–4833
Ryan B. Carnegie, Severine A. Patat and Kevin L. Schey. Proteomic Portrait of a viral response in the shrimp, Litopenaeus vannamei, gill.
In preparation, 2004
Roberts JE, Finley EL, Patat SA, Schey KL. 2001. Photooxidation of lens proteins with xanthurenic acid: a putative chromophore for cataractogenesis. Photochem Photobiol 74: 740-4
Schey KL, Patat S, Chignell CF, Datillo M, Wang RH, Roberts JE. 2000. Photooxidation of lens alpha-crystallin by hypericin (active ingredient in St. John's Wort). Photochem Photobiol 72: 200-3

ABSTRACTS:
Histones from the Pacific White Shrimp, Litopenaeus vannamei, have antimicrobial activity
Severine A. Patat, Robert Chapman, Paul S. Gross, Gregory W. Warr, and Kevin L. Schey.
NSA, Philadelphia, PA. 2005.
Identification of Post-translational Modifications of Histone Proteins by In-gel Propionylation and In-gel Digestion
Severine A. Patat and Kevin L. Schey
MUSC Student Research Day 2004.
Identification of post-translational modifications of histone proteins by in-gel propionylation and in-gel digestion
Severine A. Patat and Kevin L. Schey.
ASMS, Nashville, Tn. 2004
Histones as antimicrobial proteins in the Pacific White Shrimp Litopenaeus vannamei.
Severine A. Patat , Robert Chapman, Paul S. Gross, Gregory W. Warr and Kevin L. Schey.
Aquaculture, Hawaii, USA. 2004
Histones as antimicrobial proteins in the Pacific White Shrimp Litopenaeus vannamei.
Severine A. Patat, Robert Chapman, Paul S. Gross, Gregory W. Warr and Kevin L. Schey.
MUSC Student Research Day 2003.
Antimicrobial Activity in Oyster (Crassostra virginica) hemolymph.
J. Gordon, S. Patat and K. Schey.
MUSC Student Research Day 2003.
Proteomic Analysis of Hemolymph from Litopenaeus vannamei.
Severine A. Patat, Ryan B. Carnegie, Paul S. Gross, Gregory W. Warr and Kevin L. Schey.
MUSC Student Research Day 2002.
Proteomic Analysis of Shrimp Immunity.
Severine A. Patat, Ryan B. Carnegie, Daniel R. Knapp and Kevin L. Schey.
Southeast Regional Meeting of the American Chemical Society, Charleston, SC. 2002
Proteomic analysis of Litopenaeus vannamei response to White Spot Syndrome Virus infection.
Severine A. Patat, Ryan B. Carnegie, Paul S. Gross, Gregory W. Warr and Kevin L. Schey.
6th International Conference on Shellfish Restoration, Charleston, SC. 2002
Proteomic analysis of hemolymph from Litopenaeus vannamei infected with white spot syndrome virus
Severine A. Patat, Ryan B. Carnegie and Kevin L. Schey.
4th International Symposium on Aquatic Animal Health, New Orleans, USA. 2002.
The low molecular weight gill proteome of WSSV-challenged Litopenaeus vannamei.
Ryan B. Carnegie, Severine A. Patat and Kevin L. Schey.
4th International Symposium on Aquatic Animal Health, New Orleans, USA. 2002.
Proteomic Analysis of Gill Tissue from White Spot Syndrome Virus challenged Litopenaeus vannamei.
Ryan B. Carnegie, Severine A. Patat and Kevin L. Schey.
National Shellfisheries Association, Mystic, Co. 2002
Shrimp Protein Regulation upon Viral Infection.
Severine A. Patat and Kevin L. Schey.
MUSC Student Research Day 2001.
Mass Spectrometric Analysis of Lens Protein Phototoxidation by Hypericin (Active Ingredient in St. John's Wort).
Patat S, Schey KL, Chignell C, and Roberts JE.
International Congress of Photobiology, San Francisco, CA. 2000.