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Lisa E. Kilpatrick, Ph.D.
Research Chemist, NIST
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Research:
The focus of my research is to develop
peptide tandem mass spectral libraries and other novel bioinformatics tools
to reliably identify proteins from complex mixtures. High throughput two-dimensional separation
techniques coupled with tandem mass spectrometry are used to generate data
for analysis. Applications of this
work include identifying proteins expressed by marine organisms during disease
or in response to environmental stress.
These protein biomarkers may be used for the early detection and
treatment of disease.
Current projects include: 1)
Studying changes in protein expression in
pygmy sperm whales with cardiomyopathy. 2)
Identification of antimicrobial peptides
in blue crabs. 3)
Identifying the proteins expressed by
bacteria at elevated ocean temperatures and their role in coral disease. |
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Publications: Van Nostrand,
J. D., Arthur, J. M., Kilpatrick, L. E.,
Neely, B. A., Bertsch, P. M., and Morris, P.
J. Changes in protein expression in Burkholderia vietnamiensis
PR1301 at pH 5 and 7 with and without nickel. Microbiology,
2008, in press. Neta, P., Pu, Q. L., Kilpatrick, L., Yang, X., and Stein,
S. E. Dehydration versus deamination of N-terminal glutamine in collision-induced
dissociation of protonated peptide ions. J.
Am. Soc. Mass. Spectrom. 2007, 18, 27-36. Ervin, L. A.,
Ball, L. E., Crouch, R. K., and Schey, K. L. Phosphorylation and glycosylation
of bovine lens MP20. IOVS. 2005, 46, 627-635. Kimes, N. E., Kilpatrick,
L. E., and Morris, P. J. Temperature-dependent induction of virulence
factors in the coral pathogen Vibrio coralliilyticus. The 12th International Symposium on
Microbial Ecology, Aug. 2008. Kilpatrick, L. E., Vizcaino, M., Morris, P. J., and Stein, S. E. Analysis of the E. coli Proteome by Different 2D-LC-MS/MS Methods. The 27th International Symposiium & Exhibit on the Separation of Proteins,
Peptides, & Polynucleotides, Oct. 2007. Kilpatrick, L. E., Kimes, N., Morris, P. J., Roth, J., Rudnick,
P., Yang, X., and Stein, S. E.
Building Peptide Mass Spectral Libraries from Bacterial Proteomes
Analyzed by 2D-LC-MS/MS. The 55th
ASMS Conference on Mass Spectrometry, June 2007. Kilpatrick, L. E., Neta, P., Roth, J., and Stein, S.
E. Identification of Peptides Using
High Quality Reference MS/MS Spectra. The
53rd ASMS Conference on Mass Spectrometry, June 2005. |
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