CGS 701/702 Unit Syllabi
Quicklist
| Date |
Date |
Flex |
Instructor |
Wed Aug 24 |
1.
Molecular Structure |
Tinker Toys |
Beeson |
Thu Aug 25 |
1. Chemical Reactivity Assignment #1 Handout |
Structure & Reactivity Questions |
Beeson |
Fri Aug 26 |
1. Solvation and Electrostatics |
|
Beeson |
Mon Aug 29 |
1. Biological thermodynamics & kinetics
2. Overview of bioenergetics & redox |
|
Beeson |
Tue Aug 30 |
1.Lipids, bilayers & membranes
2.Transporters & channels |
Assignment 1 Due |
Beeson |
Wed Aug 31 |
1. Mechanisms in Glycolysis
2. Stuctural Aspects of Regulation Assignment #2 Handout |
|
Beeson |
Thu Sep 1 |
1. Electron transport chain
2. Chemiosmotic energy transduction & Oxidative hosphorylation |
|
Lemasters |
| Fri Sep 2 |
1. Beta oxidation & citric acid cycle
2. Mitochondrial physiology |
Assignment 2 Due |
Lemasters |
| Mon Sep 5 |
NO CLASS (Labor Day) |
|
|
| Tue Sep 6 |
1. Regulation of catabolism versus anabolism
2. Signaling in regulation of metabolism |
|
Beeson |
| Wed Sep 7 |
1. Probing metabolism in vitro & in vivo |
Nicholls Paper |
Beeson |
| Thu Sep 8 |
1. Metabolic Dysfunction in Disease |
Review |
Chan |
Fri Sep 9 |
UNIT EXAM |
|
|
Faculty
Craig Beeson, Ph.D., Pharmaceutical and Biomedical Sciences
Sherine Chan, Ph.D., Pharmaceutical and Biomedical Sciences
Thomas A. Dix, Ph.D., Pharmaceutical and Biomedical Sciences
John J. lemasters, MD, Ph.D., Pharmaceutical and Biomedical Sciences, Biochemistry and Molecular Biology
General Reading
|
Lehninger (4th ed) |
Stryer (7th ed) |
| Intro to Metabolism |
14 |
14 |
| Bioenergetics |
13 |
|
| Lipids, membranes |
10 |
12 |
| Ion channels |
11 |
13 |
| Glycolysis |
14, 15 |
16 |
| TCA |
16 |
17 |
| Oxidative-Phosph |
19 |
18 |
| Fatty Acid Oxidation |
17 |
22 |
| Pentose Phosphate |
15 |
20 |
Pathway (HMP-shunt)
Gluconeogenesis |
14-15 |
16 |
| Glycogen Synthesis |
15 |
21 |
| Glycogen Catabolism |
15 |
21 |
| Fatty Acid Synthesis |
17 |
22 |
Useful Websites:
http://www.bmb.leeds.ac.uk/illingworth/oxphos/
http://www.esf.edu/efb/course/EFB325/default.htm
http://www.aw-bc.com/mathews/
http://www.rpi.edu/dept/bcbp/molbiochem/MBWeb/mb1/MB1index.html
| Date |
Date |
Flex |
Instructor |
Mon Sept 12 |
Protein Folding and Stability
|
Chan & Dill Paper |
Beeson/Isaacs |
Tues Sept 13 |
Protein Modifications
|
Paper discussion |
Eblen |
Wed Sept 14 |
Protein-Protein Interactions |
Paper discussion |
Hsu |
Mon Sept 19 |
Proteomics/Informatics |
Flex activity |
Hsu |
Tues Sept 20 |
Enzyme Kinetics
|
Kinetics questions |
Beeson |
Wed Sept 21 |
Enzymes and Catalysis |
Paper discussion |
Krupenko
|
Mon Sept 26 |
Principles of X-ray Crystallography |
Flex activity |
Davies |
Tues Sept 27 |
Protein Purification |
Flex activity |
Ball |
Wed Sept 28 |
Protein Characterization/Proteins |
Paper discussion and debate |
Schwacke |
Fri Sept 30 |
Unit Exam |
|
|
Faculty
Craig Beeson, Ph.D., Pharmaceutical and Biomedical Sciences
Jennifer Isaacs, Ph.D., Pharmacology
Scott Eblen, Ph.D., Pharmacology
Yi-Te Hsu, Ph.D., Pharmacology
Chris Davies, Ph.D., Biochemistry and Molecular Biology
Lauren Ball, Ph.D., Pharmacology
Sergey Krupenko, Ph.D., Biochemistry and Molecular Biology
John Schwacke, Ph.D., Biochemistry and Molecular Biology
General reading Prerequisite readings for this Unit are Chapters 2, 3, 6, 10.3, 10.4, 23.2 and 23.3
in Stryer
6th Ed; or Chapters 2, 3, 8, 9, 10, 23.1, 23.2, 30.4 and 30.5 in Stryer 7th Ed.
All assignments are due by 10:00am on the assigned date: bring completed assignments to class. Read Assigned paers before class and be prepared to discuss them.
| Date |
Date |
Flex |
Instructor |
Mon Oct 10 |
Overview of receptors & signaling I |
Receptor tyrosine kinases |
Rosenzweig |
Tues Oct 11 |
G protein coupled
receptors |
Paper discussion (1) |
Blumer |
Wed Oct 12 |
G proteins and their regulatory proteins |
Paper discussion (2) |
Blumer |
Mon Oct 17 |
Photoreceptors |
Paper discussion (3) |
Koutalos |
Tues Oct 18 |
Protein: protein interactions in signal transduction |
Methods for identifying/studying protein interactions |
Ball |
Wed Oct 19 |
Protein kinases: MAP kinases |
Paper
discussion (5) |
Eblen |
Mon Oct 24 |
Signaling to apoptosis |
Methods
exercise |
Voelkel-Johnson
|
| Tues Oct 25 |
Cell Signaling in response to hypoxia |
Paper discussion (4) |
Isaacs |
| Wed Oct 26 |
Cell Signaling in disease: Animal models of cancer |
Paper discussion (7) |
Neumann |
Fri Oct 28 |
Unit Exam |
|
|
Faculty
Steven Rosenzweig, Ph.D., Pharmacology
Joe Blumer, Ph.D., Pharmacology
Yiannis Koutalos, Ph.D., Ophthalmology
Lauren Ball, Ph.D., Pharmacology
Scott Eblen, Ph.D., Pharmacology
Christina Voelkel-Johnson, Ph.D., Pharmacology
Jennifer Isaacs, Ph.D., Pharmacology
Carola Neumann, Ph.D., Pharmacology
General reading
Prerequisite: Chapters 15, 17 & 20 of Molecular
Biology of the Cell, 5th Ed, Alberts et al.
Specific Reading
Papers will be posted on Moodlerooms as required
Macromolecules: Nucleic Acids
Dr. Ashley Cowart
STB 421; 789-6796
cowartl@musc.edu
|
Date
|
Date
|
Flex
|
Instructor
|
|
Mon Oct 31
|
1.
Introduction to Nucleic Acids
2.
History of Nucleic Acid Research |
|
Cowart |
|
Tues Nov 1
|
1. Nucleic Acid Structure I
2. Nucleic Acid Structure II |
Hot Topic: Riboswitches
|
Hennig |
|
Wed Nov 2
|
1.
Principles of NMR Spectroscopy
2.
Nucleic Acid Structure Determination |
NMR Facility Tour
|
Hennig
|
|
Mon Nov 7
|
1. Catalytic and non-coding RNAs
2. RNA aptamers and RNA biosensors |
Paper discussion
|
Heise |
|
Tues Nov 8
|
1. Prokaryotic DNA replication
2. eukaryotic DNA replication |
Techniques used to study DNA replication
|
Mohanty |
|
Wed Nov 9
|
1. DNA damage and checkpoints
2. DNA repair and recombination
|
In unit assignment |
Mohanty |
|
Mon Nov 14
|
1. Genetics I
2. Genetics II |
In unit assignment discussion
|
Cowart |
|
Tues Nov 15
|
1. Genomics Overview
2. Genomics Information Resources |
Workshop in database searching
|
Zheng |
|
Wed Nov 16
|
Exam preparation |
|
|
|
Fri Nov 18
|
Unit Exam |
|
|
Faculty
Mirko Hennig, Ph.D., Biochemistry and Molecular Biology
Bidyut Mohanty, Ph.D.,
Biochemistry and Molecular Biology
Ashley Cowart, Ph.D., Biochemistry and Molecular Biology
Tilman Heise, Ph.D., Biochemistry and Molecular Biology
Jim Zheng, Ph.D., Biochemistry and Molecular Biology
General reading
Prerequisite: Chapters 4-7
of Molecular Biology of the Cell, 5th Ed, Alberts et al. Also useful are Chapters 4, 5, 28, 29 & 30 in
Stryer, 6th ed.
Regulation of Gene Expression
Dr. Donald Menick
Gazes/Strom Thurmond 203; 876-5045
menickd@@musc.edu
|
Date
|
Date
|
Flex
|
Instructor
|
|
Mon Nov 21
|
Molecular Biological Techniques
|
|
Kurtz
|
|
Mon Nov 28
|
1. Eukaryotic Transcription/Regulationof Gene Expression I
2. Eukaryotic Transcription/Regulation of Gene Expression II |
Discussion of Student presentations
|
Kurtz
|
|
Tues Nov 29
|
3. Integrating Transcriptional Regulation Signaling Events
4. Epigenetic Regulation of Gene Expression
|
|
Kurtz |
|
Wed Nov 30 |
5. HDAC's and HAT's Role in Gene Expression I
6. HDAC's and HAT's Role in Gene Expression II
|
|
Menick |
|
Mon Dec 5
|
7. Micro RNAs I
8. Micro RNAs II
|
TBA
|
Menick |
|
Tues Dec 6
|
9. Regulation of mRNA Processing and Localization I
10. Regulation of mRNA Processing and Localization II
|
Practice
talks
|
McDermott
|
|
Wed Dec 7
|
11. Regulation of mRNA Stability and Translation
12. Regulation of Translation and Degradation
|
Practice
talks
|
McDermott |
|
Mon Dec 12
|
Student presentations of research papers
|
|
|
| Tues Dec 13 |
Student presentations of research papers |
|
|
| Wed Dec 14 |
Student presentations of research papers |
Overview of Exam |
|
| Fri Dec 16 |
Unit Exam |
|
|
Faculty
David Kurtz, Ph.D., Pharmacology
Donald Menick, Ph.D., Medicine
Paul McDermott, Ph.D., Medicine
Gerneral Reading
Prerequisite: Chapters 7, 8 and section 6.6 of Lodish et
al, 6th Ed. Additional reading from
recent reviews will be assigned by each lecturer.
Flex-Time
Primary research papers will be presented and
discussed. Each student will select a current paper on gene expression
(instructors will provide examples). Selected papers will be submitted for Unit
leader approval by Friday, December 2, 2010. The Unit leader will give guidelines for the
presentation, and grading criteria during the first Flex-time. Students will have a practice session with a TA on December 6 or 7, 2011. This will be done with 3 students and the TA will critique each presentation. In these practice sessions students will have the opportunity to ask questions and get very good input on their presenation. Students will give their formal presentation to the entire class on December 12, 13, and 14, 2011. A secondary focus of Flex-times will be discussion of topics or techniques relevant to gene expression.
Grading
25% of the Unit
grade will derive from in-Unit research paper presentation, and 75% will derive
from the Unit exam (closed-book, in-class).
|
Date
|
Date
|
Flex
|
Instructor
|
|
Wed Jan 4
9:00-11:00am |
Lecture: Cytoskeletal Dynamics
Lecture: Extracellular Matrix |
Literature Searches
|
Krug
Bradshaw |
|
Mon Jan 9
9:00-11:00am
|
Discussion (I) Kandalam et al. (2011). Circulation 124:2094-2105
Discussion (II) Kinzel et al. (2010). Devel Cell 19:66 |
Q/A
|
Krug (I) - BE 101
Bradshaw (II) - BS435
|
|
Tues Jan 10
9:00-11:00am
|
Discussion (II) Kandalam et al. (2011). Circulation 124:2094-2105
Discussion (I) Kinzel et al. (2010). Devel Cell 19:66 |
Q/A
|
Bradshaw (II) - BS435
Krug (I) - EL 121 |
|
Wed Jan 11
9:00-11:00am
|
Lecture: Cancer and Population Dynamics
Lecture: Viral-Mediated Transformation |
Q/A
|
Gemmill
Parsons |
|
Tues Jan 17
9:00-11:00am
|
Discussion (I) Varelas et al. (2010) Devel Cell 19:831-844
Discussion (II): Gottwein and Cullen (2010).
J Virology 84:5229-5237 |
Q/A
|
Gemmill (I) - BS435
Parsons (II) - EL105
|
|
Wed Jan 18
9:00-11:00am
|
Discussion (II) Varelas et al. (2010) Devel Cell 19:831-844
Discussion (II): Gottwein and Cullen (2010).
J Virology 84:5229-5237
|
Q/A
|
Gemmill (II) - BS435
Parsons (I) - EL105 |
|
Mon Jan 23
9:00-11:00am
|
Journal Presentations |
|
Groups A & B |
|
Tues Jan 24
9:00-11:00am
|
Journal Presentations |
|
Groups C & D |
|
Wed Jan 25
9:00-11:00am
|
Review Session |
|
All instructors |
|
Fri Jan 27
9:00-11:00am
|
Unit Exam (in-class)
|
|
Krug
|
Faculty
Amy Bradshaw, Ph.D., Medicine/Cardiology
Edward Krug, Ph.D.,Unit Leader, Regenerative Medicine & Cell Biology
Bob Gemmill, Ph.D., Medicine/Hematology-Oncology
Chris Parsons, M.D., Medicine/Infectious Diseases
General Reading
Molecular Cell Biology, Lodish, 6th edition - Chapters 17 & 18, 20 & 25 (or comparable chapters in the 4th edition - online: http://www.ncbi.nlm.nih.gov/books/NBK21054
Specific Reading
Papers will be posted on Moodle
Dr. Debra Hazen-Martin
Walton Research Building 8th floor complex,
792-2906
hazenmad@@musc.edu
|
Date
|
Date
|
Flex
|
Instructor
|
|
Mon Jan 30
|
1.
Introduction
2.
Causes, mechanisms, morphology of cell and tissue injury
|
Multihead scopes with
pathologists. In-Unit assignment #1.
|
Hazen-Martin |
|
Tues Jan 31
|
1. Cellular adaptations,
cell death, and necrosis
2. Introduction to
inflammation and repair
|
Discuss and submit assignment #1 in class
|
Hazen-Martin |
|
Wed Feb 1
|
1. Inflammation/acute cell injury
2. Chemical mediators inflammation
3. Chronic inflammation/resolution |
Turn in final version assignment @1 |
Hazen-Martin |
|
Mon Feb 6
|
1. Immune System I
|
In-class assignment #2
|
Kasman
|
|
Tues Feb 7
|
1. Immune System II |
In-class activity #3;
receive assignment #4
|
Hazen-Martin (l)
Self (l) |
|
Wed Feb 8
|
Techniques Rotation Day |
Techniques Rotation
|
|
|
Mon Feb 13
|
Prepare paper discussion with group members
|
Dr. Schnellmann article discussion #4
|
|
|
Tues Feb 14
|
1. Immunopathology organ transplant rejection |
Group 1 and 2 technique presentations
|
|
|
Wed Feb 15
|
Review |
Group 3 and 4 technique presentations |
|
|
Fri Feb 17
|
Unit Exam |
|
|
Faculty
Debra Hazen-Martin, Ph.D., Pathology
Laura Kasman, Ph.D., Microbiology & Immunology
Richard Schnellmann, Ph.D., Pharmaceutical Sciences
John Metcalf, M.D., Pathology
Erika Brown, Ph.D., Pathology
General reading
Robbins Basic Pathology, 7th
edition (on reserve); the following chapters will supplement lecture notes:
|
Chapter 1:
|
Cellular Injury and response
Aging
Apoptosis
|
|
Chapter 2:
|
Inflammation |
|
Chapter 5:
|
Diseases of
Immunity |
|
Chapter 6:
|
Neoplasia |
If you prefer a more comprehensive version of this text try Robbins Pathologic Basis of Disease
which is available on line through MD Consult. Additional articles and/or
reviews may be provided in class.
Kuby's Immunology, 6th
edition (Kuby et.al. 2006) Chapters 1-10 contain basic information
that supplements lecture notes.
Specific Reading
Will be posted on Moodlerooms before the relevant lecture
and/or assignment.
|
Date
|
Date
|
Flex
|
Instructor (email)
|
|
Mon Feb 20
|
1.
Introduction to the Unit
2.
Mechanisms of Neurotransmission |
|
Halushka
Ramamoorthy |
|
Tues Feb 21
|
1. The Heart and Arterial Pressure Control Mechanisms |
|
Halushka |
|
Wed Feb 22
|
1.
Autonomic Nervous System Control of the
CV
System
|
In-Unit Assignment
|
Webb
|
|
Mon Feb 27
|
1. Molecular Basis of Atheroscierosis
|
|
Hammad |
|
Tues Feb 28
|
1. Molecular Mechanism for Asirin-mediated Cardiovascular Prophylaxis |
|
Halushka |
|
Wed Feb 29
|
1. Molecular Basis of Electrical Mechanical Coupling |
In-Unit Assignment
|
Menick |
|
Mon Mar 5
|
1. Renal Physiology |
|
Bell |
|
Tues Mar 6
|
1.Proteomic Tools for Analysis of Renal Disease |
In-Unit Assignment
|
Janech |
|
Wed Mar 7
|
1. Molecular Mechanisms of Innate Immunity |
|
Cook |
|
Fri Mar 9
|
Unit Exam |
|
|
Faculty
Perry Halushka, Ph.D., M.D., Medicine/Pharmacology
Sammanda Ramamoorthy, Ph.D., Neurosciences
Jerry Webb, Ph.D., Pharmacology
Samar Hammad, Ph.D., Medicine
Donald Menick, Ph.D., Medicine
Darwin Bell, Ph.D., Medicine
Michael Janech, Ph.D., Medicine
Jim Cook, Ph.D., Pharmacology
Chemistry of Life Processes
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