Mirko Hennig, Ph.D.
                               Assistant Professor
     
  2006 Assistant Professor, Department of Biochemistry & Molecular Biology
Medical University of South Carolina, Charleston, SC
  2002-2006 Assistant Professor – Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA.
  2001-2002 Senior Research Associate – Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA.
  1999-2001 Research Associate – Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA
  1999 Ph.D. –Johann-Wolfgang-Goethe University of Frankfurt/M, Germany.
NMR Camp 2010 - http://biochemistry.musc.edu/NMRcamp/



Office: 843-792-7336
Lab: 843-792-7918
Fax: 843-792-4322
Email: hennig@musc.edu
BSB-535D
click here for lab page

 
 

Research Interests
 

It is the goal of our research program to understand the essential features of specific, high affinity RNA recognition by proteins and small molecules. Experimentally, NMR spectroscopy combined with a wide range of biochemical and biophysical methods are used to characterize biological targets such as retroviral mRNA pseudoknots involved in ribosomal –1 frameshifting and the regulatory Rev-RRE mRNA-protein complex, important for HIV replication. Methodological developments to facilitate investigations of these macromolecular complexes by NMR spectroscopy represent another key element of our research.
 

Selected Publications

Click here to view list of publications on the National Library of Medicine's PubMed online database.
 
  • Cornish PV, Giedroc DP, and Hennig M. (2006) Dissecting Non-Canonical Interactions in Frameshift stimulating mRNA Pseudoknots. J. Biomol. NMR 35: 209-223
  • Rittershaus PC, Kechichian TB, Allegood JC, Merrill Jr. AH, Hennig M, Luberto C, and Del Poeta M. (2006) Essential role of glucosylceramide in the pathogenesis of Cryptococcus neoformans. J. Clin. Invest. 116: 1651-9
  • Hennig M, Munzarová ML, Bermel W, Scott LG, Sklenár V, and Williamson JR. (2006) Measurement of Long Range 1H-19F Scalar Coupling Constants and their Glycosidic Torsion Dependence in 5-Fluoropyrimidine Substituted RNA. J. Am. Chem. Soc. 128: 5851-8
  • Fohrer J, Hennig M, and Carlomagno T. (2006) The Influence of the 2’-Hydroxyl Group Conformation on the Stability of A-form Helices in RNA. J. Mol. Biol. 356: 280-7
  • Cornish PV, Hennig M, and Giedroc DP. (2005) A pseudoknot L2 cytidine-S1 minor groove base pairing interaction as a positive determinant for –1 ribosomal frameshift stimulation. Proc. Natl. Acad. Sci. USA 102: 12694-9
  • Plant EP, Pérez-Alvarado GC, Jacobs JL, Mukhopadhyay B, Hennig M, and Dinman JD. (2005) A three-stemmed mRNA pseudoknot in the frameshift signal of the SARS Coronavirus. PLoS Biology 3: e172
  • Hennig M, Fohrer J, and Carlomagno T. (2005) Assignment and NOE Analysis of 2’-Hydroxyl Protons in RNA: Implications for RNA Structure Stabilization. J. Am. Chem. Soc. 127: 2028-2029
  • Scott LG, Geierstanger BH, Williamson JR, and Hennig M. (2004) Enzymatic Synthesis and 19F-NMR Studies of 2-Fluoroadenine substituted RNA. J. Am. Chem. Soc. 126: 11776-11777
  • Giedroc DP, Cornish PV, and Hennig M. (2003) Detection of Scalar Couplings Involving 2’-Hydroxyl Protons Across Hydrogen Bonds in a frameshifting mRNA pseudoknot. J. Am. Chem. Soc. 125: 4676-4677
  • Nixon PL, Rangan A, Kim Y-G, Rich A, Hoffman DW, Hennig M, and Giedroc DP. (2002) Solution Structure of a luteoviral P1-P2 frameshifting RNA pseudoknot. J. Mol. Biol. 322: 621-633
  • Hennig M, Bermel W, Spencer A, Dobson CM, Smith LJ, and Schwalbe H. (1999) Side-chain conformations in an unfolded protein: chi1 distributions in denatured hen lysozyme determined by heteronuclear 13C, 15N NMR spectroscopy. J. Mol. Biol. 288: 705-23
  • Reif B, Hennig M, and Griesinger C. (1997) Direct measurement of angles between bond vectors in high-resolution NMR. Science 276: 1230-3