Eugene Cymbalyuk, Ph.D.

 

 

Post-Doctoral Fellow




 

Educational Training:
 
1987

Department of Biophysics, Lomonosov
Moscow State University

M.S. Biophysics
1992

Department of Biophysics, Lomonosov
Moscow State University

Ph.D. Biophysics


 


 

Professional Experience:     

2004-Present

Post-doctoral Fellow in the laboratory of Dr. Del Poeta

  Medical University of South Carolina, Dept of Biochemistry and Molecular Biology, Charleston, SC
 
2001-2004

Post-Doctoral Fellow in the laboratory of Dr. Yi-Te Hsu

  Medical University of South Carolina, Dept of Biochemistry and Molecular Biology, Charleston, SC
 
1996-2000

Visiting Scientist in research group of Dr. Alexander A.Bulychev, Biophysics Department, Biology Faculty,Lomonosov's Moscow State University and research group of Dr. Sergey M.Serov, Engelhardt's Institute of Molecular Biology, Russian Academy of Sciences
 

1992-1995 Post-Doctoral Fellow at joint project of Frumkin's Institute of Electrochemistry, Russian Academy of Sciences, Laboratory of Prof. Yuri A.Chizmadzhev, and Engelhardt's Institute of Molecular Biology of Russian Academy of Sciences, research group of Dr. Sergey M.Serov

 

Membership in Professional Organizations       

1994-Present Membership in Russian TeX Users Groups: CryTUG
1995-Present Membership in Russian Biochemical Society
2003-Present Membership in American Society for Cell Biology



Research Expertise:
 

Cell culture work with cell lines (CHO, HeLa, CV, COS-7, 3T3, NS0, Namalwa, Jurkat) including human and mouse fibroblast, rat neurons, mouse embryonic stem cells.  Stable and transient transfection, electroporation.

Fluorescent microscopy.  Molecular biology techniques for DNA, RNA Proteins and Lipids analysis.  Protein expression and purification.  Protein and lipid biochemistry



Publications
: 

1.

Cymbalyuk Eugene, Qi Hou, and Yi-Te Hsu. Apoptosis associated with the transient expression of Bcl-2 involves primarily the synergistic action of its flexible loop and C-terminal segment. - J. Biol.Chem., on revision
 

2.

Hou Q., Cymbalyuk E., Hsu S.C., Xu M., and Y.T. Hsu. Apoptosis modulatory activities of transiently expressed Bcl-2: roles in cytochrome C release and Bax regulation. - Apoptosis, 2003, v. 8, n. 6, 617-629
 

3.

Hsu Y.T., Hou Q, Cymbalyuk, E., and R. Youle. Generation and characterization of species-specific anti-Bcl-XL monoclonal antibodies. - Hybrid Hybridomics. 2003, n. 2, 91-95
 

4.

Makarova I.V., Neznanov N.S., Tsymbaliuk E.S. (a variant of my name spelling), Andreeva L.Ye., Manuilova Ye.S., Grivennikov I.A., Tarantul V.Z., Serov S.M., Adamson E.D. and K.G. Gazaryan. Targeting of platelet-derived growth factor A gene and generation of a chimeric mouse with the knockout gene. - Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (Mosc.), 1997, n. 4, pp. 23-25
 

5.

Abramyan L.G., Tsymbaliuk E.S. (a variant of my name spelling), Gazaryan T.G., Levchenko I.V., Andreeva N.B. and K.G. Gazaryan. Expression of Escherichia coli homoserine kinase gene in mammalian cells. - Molekulyarnaya Biologiya (Mosc.), 1996, v. 30, n. 4, pp. 838-845.
 

6.

Cymbalyuk E.S. and S.M. Serov. Transformation of immobilized cells at two-pulse electrotreatment with addition of donor DNA between pulses. - Biol. Membr. (Mosc.), 1993, v. 10, n. 6, pp. 581-586
 

7.

Bulychev A.A., Cymbalyuk E.S. and E.P. Lukashev. Use of irreversible electroporation and osmotic effects to prove photoinduced accumulation of methylphenazonium cations in the internal volume of thylakoids. - Biol. Membr. (Mosc.), 1993, v. 10, n. 6, pp. 587-597
 

8.

Sabelnikov A.G., Cymbalyuk E.S., Gongadze G. and V.L. Borovyagin. Escherichia coli membranes during electrotransformation: an electron microscopy study. - Biochim. Biophys. Acta, 1991, v. 1066, n. 1, pp. 21-28
 

9.

Cymbalyuk E.S. and A.G. Sabelnikov. Agarose gel technique to study the mechanisms of electrotransformation of Escherichia coli cells. - Biol. Membr. (Mosc.), 1991, v. 8, n. 4, p. 445-447
 

10.

Sabelnikov A.G. and E.S. Cymbalyuk. Clotting of donor DNA during electroporation of procaryotes. - Bioelectrochem. Bioenerg., 1990, v. 24, n. 3, pp. 313-321
 

11.

Cymbalyuk E.S., Chernomordik L.V., Broude N.E. and Yu.A. Chizmadzhev. Electro-stimulated transformation of E.coli cells pretreated by EDTA solution. - FEBS Lett., 1988, v. 234, n. 1, pp. 203-207
 

12.

Cymbalyuk E.S., Chernomordik L.V., Broude N.E. and Yu.A. Chizmadzhev. Electro-stimulated transformation of Escherichia coli cells. - Biol. Membr. (Mosc.), 1988, v. 5, n. 3, pp. 240-245
 

 
Abstracts:
 

Cymbalyuk Eugene, Qi Hou, and Yi-Te Hsu. Transient Overexpression of Bcl-2 Promotes Apoptosis: Roles of Cytochrome C Release and Bax Regulation, and Identification of the Pro-apoptotic Domains. The 43rd American Society for Cell Biology, Annual Meeting 2003, Poster 03-LB-3616-ASCB

Cymbalyuk E.S., and S.M. Serov. Low field electrotransfection of the preelectroporated mammalian cells. Proc. 23rd FEBS Meeting, 1995, P37.21, p. 241.
 

 

 

 

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