
Professional
Experience:
|
2004-Present |
Post-doctoral Fellow in the
laboratory of Dr. Del Poeta |
| |
Medical University
of South Carolina, Dept of Biochemistry and Molecular
Biology, Charleston, SC
|
|
2001-2004 |
Post-Doctoral Fellow in the laboratory of Dr. Yi-Te
Hsu |
| |
Medical University
of South Carolina,
Dept of
Biochemistry and Molecular Biology, Charleston, SC
|
|
1996-2000 |
Visiting Scientist in research
group of Dr. Alexander A.Bulychev, Biophysics Department, Biology
Faculty,Lomonosov's Moscow State University and research group of Dr.
Sergey M.Serov, Engelhardt's Institute of Molecular Biology, Russian
Academy of Sciences
|
|
1992-1995 |
Post-Doctoral Fellow at joint project
of Frumkin's Institute of Electrochemistry, Russian Academy of Sciences,
Laboratory of Prof. Yuri A.Chizmadzhev, and Engelhardt's Institute of
Molecular Biology of Russian Academy of Sciences, research group of Dr.
Sergey M.Serov |
Membership in
Professional Organizations:
| 1994-Present |
Membership in Russian
TeX Users Groups: CryTUG |
| 1995-Present |
Membership in Russian
Biochemical Society |
| 2003-Present |
Membership in
American Society for Cell Biology |
Research Expertise:
|
Cell culture work with cell lines (CHO,
HeLa, CV, COS-7, 3T3, NS0, Namalwa, Jurkat) including human and
mouse fibroblast, rat neurons, mouse embryonic stem cells.
Stable and transient transfection, electroporation. |
|
Fluorescent microscopy.
Molecular biology techniques for DNA, RNA Proteins and Lipids
analysis. Protein expression and purification.
Protein and lipid biochemistry |
Publications:
|
1. |
Cymbalyuk
Eugene, Qi Hou, and Yi-Te Hsu. Apoptosis associated with the
transient expression of Bcl-2 involves primarily the synergistic
action of its flexible loop and C-terminal segment. - J.
Biol.Chem., on revision
|
|
2. |
Hou Q., Cymbalyuk E., Hsu S.C., Xu M., and Y.T. Hsu.
Apoptosis modulatory activities of transiently expressed Bcl-2:
roles in cytochrome C release and Bax regulation. - Apoptosis,
2003, v. 8, n. 6, 617-629
|
|
3. |
Hsu Y.T., Hou Q, Cymbalyuk, E., and R. Youle.
Generation
and characterization of species-specific anti-Bcl-XL monoclonal
antibodies. - Hybrid Hybridomics. 2003, n. 2, 91-95
|
|
4. |
Makarova I.V., Neznanov N.S., Tsymbaliuk
E.S. (a variant of my name spelling), Andreeva L.Ye., Manuilova
Ye.S., Grivennikov I.A., Tarantul V.Z., Serov S.M., Adamson E.D.
and K.G. Gazaryan.
Targeting of platelet-derived growth factor A
gene and generation of a chimeric mouse with the knockout gene.
- Molekulyarnaya Genetika Mikrobiologiya i Virusologiya (Mosc.),
1997, n. 4, pp. 23-25
|
|
5. |
Abramyan
L.G., Tsymbaliuk E.S. (a variant of my name spelling),
Gazaryan T.G., Levchenko I.V., Andreeva N.B. and K.G. Gazaryan.
Expression of Escherichia coli homoserine kinase gene in
mammalian cells. - Molekulyarnaya Biologiya (Mosc.), 1996, v.
30, n. 4, pp. 838-845.
|
|
6. |
Cymbalyuk
E.S. and S.M. Serov. Transformation of immobilized cells at
two-pulse electrotreatment with addition of donor DNA between
pulses. - Biol. Membr. (Mosc.), 1993, v. 10, n. 6, pp. 581-586
|
|
7. |
Bulychev A.A., Cymbalyuk E.S. and E.P. Lukashev. Use of
irreversible electroporation and osmotic effects to prove
photoinduced accumulation of methylphenazonium cations in the
internal volume of thylakoids. - Biol. Membr. (Mosc.), 1993, v.
10, n. 6, pp. 587-597
|
|
8. |
Sabelnikov A.G., Cymbalyuk E.S., Gongadze G. and V.L.
Borovyagin.
Escherichia coli membranes during electrotransformation: an electron microscopy study. - Biochim.
Biophys. Acta, 1991, v. 1066, n. 1, pp. 21-28
|
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9. |
Cymbalyuk E.S. and A.G. Sabelnikov.
Agarose gel technique to study the mechanisms of
electrotransformation of Escherichia coli cells. - Biol. Membr.
(Mosc.), 1991, v. 8, n. 4, p. 445-447
|
|
10. |
Sabelnikov A.G. and E.S. Cymbalyuk.
Clotting of donor DNA
during electroporation of procaryotes. - Bioelectrochem.
Bioenerg., 1990, v. 24, n. 3, pp. 313-321
|
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11. |
Cymbalyuk
E.S., Chernomordik L.V., Broude N.E. and Yu.A. Chizmadzhev.
Electro-stimulated transformation of E.coli cells pretreated by
EDTA solution. - FEBS Lett., 1988, v. 234, n. 1, pp. 203-207
|
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12. |
Cymbalyuk
E.S., Chernomordik L.V., Broude N.E. and Yu.A. Chizmadzhev.
Electro-stimulated transformation of Escherichia coli cells. -
Biol. Membr. (Mosc.), 1988, v. 5, n. 3, pp. 240-245
|
Abstracts:
|
Cymbalyuk
Eugene, Qi Hou, and Yi-Te Hsu. Transient Overexpression of Bcl-2
Promotes Apoptosis: Roles of Cytochrome C Release and Bax
Regulation, and Identification of the Pro-apoptotic Domains. The
43rd American Society for Cell Biology, Annual Meeting 2003,
Poster 03-LB-3616-ASCB |
|
Cymbalyuk E.S., and S.M. Serov. Low field electrotransfection of the
preelectroporated mammalian cells. Proc. 23rd FEBS Meeting, 1995,
P37.21, p. 241.
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