Table of Contents

Nos.	Title (Contributor)

0001	E. coli Transformation for Subcloning (NC)
0002	DNA Preparation from 10-mL Yeast Culture (NC)
0003	Direct PCR from Whole Yeast Cells: Zymolyase Method (NC)
0004	Phosphate Assay by Jiandi Zhang (JZ)
0005	Trypsinization of Adherent Cells (SJ)
0006	Freezing Cells (SJ)
0007	Thawing cells (SJ)
0008	Selection of Transfected Suspension Cells (SJ)
0009	Apoptosis: Miniassay (SJ)
0010	Flow Cytometric Analysis of Cell Cycle (SJ)

0011	Cell Disruption and Subcellular Fractionation (SJ)
0012	Mycoplasma Testing with MycoTect Kit (SJ)
0013	NBT assay (SJ)
0014	NBT assay: Brice Weinberg protocol (SJ)
0015	Differentiation with inducers for NBT (SJ)
0016 	Percoll Gradient (SJ)
0017	Proliferation Assay: [3H] Thymidine Incorporation (SJ)
0018	Preparation of TNFa (SJ)
0019	Biorad Protein Assay: Bradford (SJ)
0020	DEAE Column (SJ)

0021	DEAE column: FPLC (SJ)
0022	Desalting Acidic Glycosphingolipids (SJ)
0023	Arachidonic Acid Labeling (SJ)
0024	AA & Metabolite Quantitation of Cell Pellets Post-AA Labeling (SJ)
0025	AA & Metabolite Quantitation of Media Post-AA Labeling (SJ)
0026	Neutralizing Arachidonic Acid (SJ)
0027	Preparation of bOG-DOPG Mixed Micelles (SJ)
0028	[3H] Choline Labeling and TNF Treatment of HL-60 Cells (SJ)
0029	DGK Assay (SJ)
0030	DGK Membrane Preparation (SJ)

0031	Column Purification of Demethylated Sphingomyelin (SJ)
0032	Purification of Demethylated Sphingomyelin (SJ)
0033	Drying Lipids with Liquid Nitrogen Evaporator (SJ)
0034	Glycosphingolipid analysis (SJ)
0035	In vitro Sphingomyelinase Assay (SJ)
0036	Labelled Sphingmyelin Synthesis (SJ)
0037	After Using the Nitrogen Evaporator (SJ)
0038	Phosphate Assay by Suprya Jayadev (SJ)
0039	Purification of Demethylated Sphingomyelin (SJ)
0040	Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells (SJ)

0041	Sphingomyelin Mass Measurement (SJ)
0042	Thin Layer Chromatography (SJ)
0043	cDNA Synthesis from MOLT-4 Cells(CM)
0044	mRNA Purification from MOLT-4 Cells (CM)
0045	How to Import Medline Search Result in Reference Manager (NC)
0046     Yeast Strain: made with FileMarkerPro 3.0 on Mac (NC)
0047     3-D Structure of Sphingolipid Metabolites (NC)
0048     Vector List for Hannun/Obeid Lab (NC)
0049     RNAi (BP)
0050     IPS-PLC (YO & SV)
0051     Labeling study: cold-inositol (YO & SV)
0052     Labeling study: inositol  (YO & SV)
0053     In vitro Ceramidase Assay (SE)
0054     In vitro Reverse Ceramidase Assay (SE)

0055     Working With Lipids And Membranes In BIACORE
        (Standard Operating Procedure) (JJ)
0056     Lipid-Protein Overlay Assay (Fat Blotting)  (JJ)
0057     Sucrose-loaded Vesicle Assay (JJ)