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Differentiation with Inducers for NBT
Contributor: Suprya Jayadev
Date: January 10, 1991
1) Start with half a flask of cells at ~1 X 106 cells/ml.
2) Sterilly wash with room temperature PBS 1-2X.
3) Resuspend pellet in ~10 ml of sterile serum free media and count cells.
4) Increase volume to yield 2 X 105 cells/ml.
5) Add serum supplement (insulin, transferrin, sodium selinite) to a final
concentration of 6 ml/L.
6) Aliquot 5 mls to each well of a 6 well CoStar plate and add appropriate
treatments.